DNA Barcoding of Koreanohadra kurodana(Gastropoda: Camaenidae)
Kang-San Kim1, Jun-Sang Lee2, Kuem Hee Jang1, *
1 Insect & Invertebrate Research Team, Research Center for Endangered Species, National Institute of Ecology, Yeongyang 36531, Korea
2 Korea Mollusca Research Institute, Chuncheon 24399, Korea
The land snail, Koreanohadra kurodana (Pilsbry, 1926), is endemic to Korea and was collected from Pocheon, Pyeongchang, and Taebaek in South Korea. This study describes the mitochondrial cytochrome c oxidase subunit I gene (COI) and 16S ribosomal RNA (16S) sequences of K. kurodana, followed by an analysis of the genetic distance between the genus Koreanohadra and its congeners. As a result, intra-species variation was 0.2-3.7% in COI and 0.3-4.0% in 16S. In the gene sequences of K. koreana recently reported by Japanese researchers, both COI and 16S sequences were observed to be located within the intra-species variation of K. kurodana (1.5-3.5% in COI; 0.6-4.0% in 16S). The inter-generic variation between genera Karaftohelix and Koreanohadra, closely related, was 11.8-26% and 6.2-18.7% in COI and 16S, respectively.
Camaenidae, COI, Korean Peninsula, land snail, 16S